Table 1.

Amino Acids in Apoplasmic Wash Fluids of lht1-lht1 and Wild-Type Plants

Amino AcidsCol-0 (n = 3)lht1/lht1 (n = 3)Change (%)P
Asp263.7 ± 37.9220.4 ± 8.4−16.4n.s
Thr172.6 ± 24.867 ± 82.1−61.2n.s.
Ser373.3 ± 47.2834.3 ± 60.4+123.4<0.01
Asn73.8 ± 4.375.4 ± 53.8+2.1n.s.
Glu414.8 ± 66.7416.5 ± 13.2+0.4n.s.
Gln582.2 ± 85.4790.3 ± 112.4+35.8n.s.
Pro124.2 ± 12.791.2 ± 10.2−26.6n.s.
Gly83.4 ± 9.4105.9 ± 7.4+26.9n.s.
Ala702.7 ± 109.6814.8 ± 113.5+16n.s.
Val48.7 ± 10.8123.9 ± 5.5+154.6<0.01
Ile22.8 ± 1.965.9 ± 9.3+189.8<0.01
Leu17 ± 5.278.2 ± 4.8+361.3<0.001
Phe12 ± 7.464 ± 9.3+433.6<0.01
GABA278.6 ± 46.6378.1 ± 46.5+35.7n.s.
Lys26.9 ± 2.662.3 ± 2.6+131.6<0.001
His26.0 ± 8.146.6 ± 4.2+79.6<0.05
Arg57.6 ± 7.992 ± 21.1+59.9n.s.
Total3,280 ± 4884,326 ± 546+32<0.05
  • Amino acids were determined after infiltration and centrifugation of leaves of 3 × 30 wild-type plants and 3 × 100 mutant plants (see Methods). The concentrations of 14 amino acids were increased, and those of 3 amino acids were decreased; the levels of 7 amino acids were increased significantly, including Ser. Total concentration of amino acids was also significantly increased in the mutants. Student's t test was used for statistical analysis. Values shown are μM ± se. n.s., not significant.