Table 2.

Metabolic Analysis of Leaves of the T-DNA Insertion Line Salk_034566 (lht1/lht1) Compared with the Wild Type (Col-0)

AnalysisCol-0 (n = 7)lht1/lht1 (n = 15)Change (%)P
Amino Acids
    Asp18.4 ± 0.817.2 ± 0.67−6.3n.s.
    Thr8.2 ± 0.48.9 ± 0.31+8.9n.s.
    Ser12.5 ± 0.923.7 ± 0.79+89.2<0.001
    Asn4.5 ± 0.3212.8 ± 0.71+186.2<0.001
    Glu41.7 ± 1.633.9 ± 1.3−18.7<0.01
    Gln30.5 ± 2.423.6 ± 1.0−22.7<0.01
    Pro7.6 ± 0.32.7 ± 0.19−64.1<0.001
    Gly0.79 ± 0.060.83 ± 0.06+3.9n.s.
    Ala5.9 ± 0.185.8 ± 0.25−2.6n.s.
    Citrulline0.56 ± 0.040.39 ± 0.04−29.9<0.05
    Val2.86 ± 0.124.3 ± 0.13+52.0<0.001
    Cys0.48 ± 0.040.81 ± 0.03+69.4<0.001
    Met0.09 ± 0.010.11 ± 0.01+19.7<0.05
    Ile0.50 ± 0.040.81 ± 0.05+60.6<0.001
    Leu0.62 ± 0.050.88 ± 0.05+41.8<0.01
    Tyr0.16 ± 0.030.19 ± 0.02+17.9n.s.
    Phe0.42 ± 0.041.6 ± 0.08+274.9<0.001
    GABA0.42 ± 0.050.4 ± 0.02−1.7n.s.
    Ethanolamine1.34 ± 0.121.7 ± 0.09+26.9<0.05
    Lys0.56 ± 0.040.95 ± 0.04+69.8<0.001
    His0.51 ± 0.031.0 ± 0.04+103.1<0.001
    Arg1.85 ± 0.193.7 ± 0.3+101.3<0.01
    Total145.2 ± 4.7151.3 ± 4.8+4.20n.s.
Anions
    Fluoride27.8 ± 2.025.3 ± 1.06−9.1n.s.
    Chloride41.7 ± 3.543.7 ± 0.89+4.8n.s.
    Nitrate1604.4 ± 42.6777.7 ± 22.2−51.5<0.001
    Phosphate137.5 ± 4.8171.1 ± 5.4+24.5<0.01
    Sulfate193.7 ± 6.7219.3 ± 5.6+13.2<0.05
    Total2,005.2 ± 49.11,237.2 ± 26.8−38.3<0.001
Cations
    Sodium44.5 ± 1.543.1 ± 2.1−3.2n.s.
    Ammonium81.8 ± 20.6235.2 ± 25.7+187.5<0.001
    Potassium936.7 ± 28.7885.6 ± 25.1−5.5n.s.
    Magnesium211.3 ± 5.7132.4 ± 3.2−37.7<0.001
    Calcium1110.2 ± 26.8637.5 ± 15.2−42.6<0.001
    Total2,384.6 ± 53.31,933.9 ± 50.9−18.9<0.001
Soluble protein102.2 ± 3.4118.4 ± 2.4+15.8<0.01
  • Rosette leaves were pooled and homogenized in liquid nitrogen, and samples were freeze-dried before analysis. Sixteen of 22 determined amino acids (including GABA) were significantly changed. The strongest changes among anions and cations were reduced nitrate and increased ammonium content in lht1/lht1 plants. Total protein content was significantly increased (+15%) in the insertion lines. Student's t test was used for statistical analysis. Values shown are nmol/mg dry weight ± se except for soluble protein, which is μg/mg dry weight. Also for soluble protein, n = 5 for Col-0 and n = 6 for lht1/lht1. n.s., not significant.