Table 3.

Amino Acids in Apoplasmic Wash Fluids of P35S-LHT1 and Wild-Type Plants

Amino AcidsCol-0 (n = 3)P35S-LHT1 (n = 3)Change (%)P
Asp263.7 ± 37.9302.9 ± 49.5+14.9n.s.
Thr + Ser546.1 ± 42.4586.3 ± 59.3+7n.s.
Asn73.8 ± 4.331.6 ± 20.6−57.2n.s.
Glu414.8 ± 66.7438.2 ± 41.0+5.6n.s.
Gln582.2 ± 85.4611.9 ± 55.5+5n.s.
Pro124.2 ± 12.7189.1 ± 7+52.3<0.01
Gly77.9 ± 9.4180.8 ± 105.1+132n.s.
Ala669.9 ± 109.6770.5 ± 107.1+15.1n.s.
Val48.7 ± 10.836.4 ± 5.6−25.1n.s.
Ile22.8 ± 1.915.4 ± 2.5−32.4n.s.
Leu17 ± 5.220.5 ± 3+21.2n.s.
Phe12 ± 7.419.5 ± 4.1+62.9n.s.
GABA278.6 ± 46.6290.5 ± 43.8+4.3n.s.
Lys26.9 ± 2.620.8 ± 6.3+22.7n.s.
His22.5 ± 8.123.5 ± 4+4n.s.
Arg57.6 ± 7.927.8 ± 2−52<0.01
Total3,237 ± 2493,565 ± 317+10n.s.
  • Amino acids were determined after infiltration and centrifugation of leaves. Total concentration of amino acids was not significantly changed; only Pro concentration was significantly increased in the P35S-LHT1 plants. Student's t test was used for statistical analysis. Values shown are μM ± se. n.s., not significant.