Table 1.

Microinjection Studies on KN1 Movement in the Presence of MPB2C

Injected ProbesInjectionsMovement (%)Comment
Lucifer yellow CH2020 (100)Extensive; cells symplastically connected
Acridine orangea2020 (100)Nuclei, extensive; cells symplastically connected
12-kD F-dextran + 11-kD rhodamine-dextran201 (5)Both dyes remain in injected cells
Rhodamine-KN1109 (90)Extensive
Rhodamine-KN1 + 12-kD F-dextran129 (75)Both extensive
FITC-Nt MPB2C100 (0)Appears in punctae and cytosol
FITC-At MPB2C100 (0)Appears in punctae and cytosol
Nt MPB2C + 12-kD F-dextran100 (0)No increase of PD SEL; dye remains in injected cells
At MPB2C + rhodamine-KN1 + 12-kD F-dextran100 (0)No increase of PD SEL; dye remains in injected cells
FITC-At MPB2C + rhodamine-KN1120 (0)Appears in punctae and cytosol
At MPB2C + TMV MP + 12-kD F-dextran240 (0)No increase of PD SEL; dye remains in injected cells
At MPB2C + CMV MP + 12-kD F-dextran1815 (84)Extensive; increase of PD SEL
  • Fluorescent probes were microinjected into N. benthamiana mesophyll cells and distribution was evaluated within 5 min. Equimolar amounts were used in probes in which two proteins were combined and adjusted to a final concentration of 2 μg/μL.

  • a Acridine orange (molecular weight = 302).