Table 2.

Putative DELLA Targets

Microarray DataqRT-PCR DatabChIP-qPCRc
AGI LocusaDescriptionaDEX-(rga-Δ17) 2 h DEX 4 h DEXga1-3 1 h GAga1-3 1 h GAga1-3 3 h GAPRGA:(rga-Δ17)dFold EnrichmentP Valuee
1At5g51810GA20ox24.345.4−10.6−4.4 ± 0.3−23.5 ± 3.56.5 ± 0.01.0 ± 0.10.521
2At1g15550GA3ox11.02.4−10.9−7.1 ± 0.6−18.0 ± 3.66.3 ± 0.21.4 ± 0.30.287
3At3g05120AtGID1a1.31.7−4.2−2.1 ± 0.4−2.3 ± 0.12.0 ± 0.22.1 ± 0.40.043
4At3g63010AtGID1b2.94.3−5.1−2.9 ± 0.2−2.5 ± 0.63.1 ± 0.32.1 ± 0.20.019
5At3g11280MYB2.42.6−1.4−1.3 ± 0.0−2.1 ± 0.22.1 ± 0.02.7 ± 0.10.002
6At5g50915bHLH137fg4.710.0−1.6−1.4 ± 0.1−6.7 ± 1.83.1 ± 0.21.8 ± 0.40.087
7At2g31730bHLH154g2.02.0−1.4−1.3 ± 0.0−2.2 ± 0.21.8 ± 0.22.1 ± 0.70.228
8At5g52830WRKY27f4.65.0−1.6−1.6 ± 0.1−1.4 ± 0.12.0 ± 0.11.3 ± 0.10.058
9At1g50420SCL34.06.4−6.8−7.7 ± 0.4−8.2 ± 1.42.8 ± 0.43.5 ± 0.2<0.0001
10At1g67100LBD40f3.16.9−2.2−2.2 ± 0.7−9.3 ± 0.93.9 ± 0.72.0 ± 0.10.011
11At4g23060IQD22f3.92.6−2.8−3.0 ± 0.2−2.3 ± 0.42.6 ± 0.22.3 ± 0.80.152
12At5g67480BT4h1.71.4−2.0−2.2 ± 0.2−2.2 ± 0.31.3 ± 0.4ND
13At2g45900Exp-PT131.531.8−13.5−7.9 ± 1.3−10.8 ± 0.45.9 ± 0.81.9 ± 0.60.243
14At2g34340Exp-PT2h1.33.7−1.4−1.7 ± 0.2−2.2 ± 0.21.2 ± 0.1ND
15At4g36410UBC17h12.817.0−4.1−1.8 ± 0.1−4.1 ± 0.7−1.4 ± 0.0ND
16At2g04240XERICO1.91.7−3.7−2.4 ± 0.3−3.0 ± 0.51.9 ± 0.11.3 ± 0.10.080
17At4g19700RING1.71.9−5.5−2.4 ± 0.4−2.8 ± 0.92.8 ± 0.11.4 ± 0.30.262
18At3g52870CaM-BPi−1.8−1.6−1.5−1.1 ± 0.01.0 ± 0.31.2 ± 0.0ND
  • a The genes in boldface are responsive to both GA and DELLA, determined by microarray and qRT-PCR analyses.

  • b Average values of three repeats ± se.

  • c Means of three independent ChIP-qPCR ± se. ND, not determined.

  • d FC between the Ler/PRGA:(rga-Δ17) transgenic line and wild-type Ler.

  • e A t test analysis was performed using the statistical package SAS 9.1.3.

  • f Genes that do not overlap between the ga1-3 and the DEX-(rga-Δ17) microarray data sets but that were rescued after further testing by qRT-PCR (see text).

  • g Name according to Bailey et al. (2003).

  • h GA-responsive genes with weak response to DELLA, measured by qRT-PCR.

  • i This gene is considered a false positive because its expression, when measured by qRT-PCR, was not responsive to GA or DELLA.