Table 1.

A Comparison of Labeling Methods for MS-Based Quantitative Proteomics

Label MethodIn VivoIn VitroMS ModeMS/MS ModeUsed in Plants
15N metabolic labeling××Ippel et al. (2004); Whitelegge (2004); Whitelegge et al. (2004); Engelsberger et al. (2006); Benschop et al. (2007); Huttlin et al. (2007)
SILAC××Gruhler et al. (2005)
18O-trypsin××Nelson et al. (2006)
ICAT××Islam et al. (2003); Dunkley et al. (2004); Majeran et al. (2005); Hartman et al. (2007)
iTRAQ××Dunkley et al. (2006); Jones et al. (2006); Rudella et al. (2006); Nühse et al. (2007); Patterson et al. (2007)
Label-free××Chen et al. (2005) (2007); Majeran et al. (2005); Niittyla et al. (2007)
AQUA××Glinski et al. (2003); Glinski and Weckwerth (2005)