Table 2.

Two- and Three-Hybrid Interactions of MADS Box Proteins of Wheat in the Yeast GAL4 System

AD\BDWAP1WAP3/WPI-1WAP3/WPI-2WAG-1WAG-2WLHS1-AWLHS1-BWLHS1-DWSEP-AWSEP-BWSEP-D
WSEP-A++++++++++++
WSEP-B++++++++++++
WSEP-D++++++++++++
WLHS1-A
WLHS1-B+++++++++
WLHS1-D+++++++++
  • Protein interactions are assessed by the viability of yeast transformants on selective medium. Relative levels of protein interaction denoted by the following: ++, strong; +, moderate; −, not detectable. AD indicates a chimeric protein with the transcriptional activation domain, and BD indicates a chimeric protein with the DNA binding domain.