Table 2.

Transmission Efficiency Test through Reciprocal Crosses

Parental Genotypes (Female × Male)Progeny
Crossrhf1a/RHF1aRHF1a/RHF1arhf2a/RHF2aRHF2a/RHF2aTotalTEFTEM
RHF1a/rhf1a rhf2a/rhf2a × wild type10414324724772.7%NA
Wild type × RHF1a/rhf1a rhf2a/rhf2a117136253253NA86.0%
rhf1a/rhf1a RHF2a/rhf2a × wild type26212114126285.8%NA
Wild type × rhf1a/rhf1a RHF2a/rhf2a257110147257NA74.8%
  • Reciprocal crosses among wild-type plants, RHF1a/rhf1a rhf2a, and rhf1a RHF2a/rhf2a were used to determine the transmission efficiency of gametes from RHF1a/rhf1a rhf2a and rhf1a RHF2a/rhf2a. Because of the loss of the kanamycin selection marker for the SALK mutants, we used PCR to determine the transmission efficiencies (TE). TE is calculated according to the following: TE = number of progenies with T-DNA insertion/number of progenies without T-DNA insertion × 100%. 1:1 for the reciprocal crosses is the expected value for the normal gamete transmission. TEF, female transmission efficiency; TEM, male transmission efficiency; NA, not applicable.