Table 1.

Relative Amounts of N-Glycans in Leaves from Wild-Type, alg3-2, and Complemented alg3-2 Plants

Wild Typealg3-2alg3-2ALG3 YFP
YoungOldOld +ManIYoungOldOld +ManI
m/z (M + Na)+Hybrid- and Complex-Type Structures% of Total% of Total% of Total% of Total% of Total% of Total% of Total
1065.4Man3XylGlcNAc28.35.01.76.87.34.74.7
1211.4Man3XylFucGlcNAc215.311.313.116.119.521.720.2
1268.5GlcNAcMan3XylGlcNAc28.57.73.55.86.84.62.2
1414.5GlcNAcMan3XylFucGlcNAc28.79.112.510.611.917.110.8
1617.6GlcNAc2Man3XylFucGlcNAc215.115.023.718.716.727.316.8
(High) Mannose-Type Structures
933.3Man3GlcNAc2 (Man3*)NDNDND24.621.122.1ND
1095.4Man4GlcNAc2 (Man4*)NDNDND11.811.4NDND
1257.4Man5GlcNAc2 (Man5/Man*)26.731.745.55.65.42.621.6
1419.5Man6GlcNAc24.77.5NDNDNDND7.6
1581.6Man7GlcNAc24.55.7NDNDNDND6.5
1743.6Man8GlcNAc25.95.2NDNDNDND5.8
1905.7Man9GlcNAc22.21.8NDNDNDND2.9
  • The percentage is the total peak area divided by specific peak areas in MALDI-TOF mass spectra. +ManI, treated with α(1-2)-mannosidase; m/z, mass-to-charge ratio; ND, not detectable.