Table 2.

Relative Amounts of N-Glycans in Leaves of cgl and alg3-2 cgl Double Mutant Plants

cglalg3-2 cglalg3-2 cgl
m/z (M + Na)+Hybrid- and Complex-Type Structures% of Total% of Total% of Total
1065.4Man3XylGlcNAc2NDNDND
1211.4Man3XylFucGlcNAc2NDNDND
1268.5GlcNAcMan3XylGlcNAc2NDNDND
1414.5GlcNAcMan3XylFucGlcNAc2NDNDND
1617.6GlcNAc2Man3XylFucGlcNAc2NDNDND
(High) Mannose-Type Structures
933.3Man3GlcNAc2 (Man3*)ND78.498.8
1095.4Man4GlcNAc2 (Man4*)ND15.3ND
1257.4Man5GlcNAc2 (Man5/Man5*)84.46.41.2
1419.5Man6GlcNAc25.0NDND
1581.6Man7GlcNAc24.3NDND
1743.6Man8GlcNAc25.8NDND
1905.7Man9GlcNAc20.5NDND
  • The percentage is the total peak area divided by specific peak areas in MALDI-TOF mass spectra. +ManI, treated with α(1-2)-mannosidase; ND, not detectable.