Primer List
| Gene Name | Forward Primers (FP) | Reverse Primers (RP) |
|---|---|---|
| RT-PCR and genotyping PCR | ||
| PUB22 | ||
| 22 FP1 and 22 RP1 | 5′-GTTTAGATTCGTTCGTCGTTTC-3′ | 5′-ACTGACACATGTCGTTGAGATTC-3′ |
| 22 FP2 and 22 RP2 | 5′-GAATCTCAACGACATGTGTCAGT-3′ | 5′-AAGCAGGATACGAATCATACAAA-3′ |
| 22 FP3 and 22 RP3 | 5′-GCTTGAACAACGTACATGTTTG-3′ | 5′-CTACACATAATGATAAACATTGAAAGA-3′ |
| PUB23 | ||
| 23 FP1 and 23 RP1 | 5′-GATCAATATATTCGTCAGGGTTC-3′ | 5′-CCCATTTTCTGAGTCGTTGC-3′ |
| 23 FP2 and 23 RP2 | 5′-GCAACGACTCAGAAAATGGG-3′ | 5′-CATCAGCAGGGATATGCAAG-3′ |
| 23 FP3 and 23 RP3 | 5′-AACAATACAAACAAGTCCAATTCTC-3′ | 5′-AGCCTGGTAACTTGTGATTTTAT-3′ |
| RD29a | 5′-CAGGTGAATCAGGAGTTGTT-3′ | 5′-CCGGAAATTTATCCTCTTCT-3′ |
| RD22 | 5′-GCAGCGAAGGAGACTCAGCT-3′ | 5′-GTTCCAAGCTGAGGTGTTCTT-3′ |
| 18s rRNA | 5′-GCATTTGCCAAGGATGTTTT-3′ | 5′-GTACAAAGGGCAGGGACGTA-3′ |
| UBC10 | 5′-ATGGCGTCGAAGCGGATC-3′ | 5′-TTAGCCCATGGCATACTTCTG-3′ |
| Ub | 5′-ATGCAGATCTTTGTTAAGACTCTC-3′ | 5′-TCAACCACCACGGAGCCTGA-3′ |
| Site-directed mutagenesis | ||
| PUB22 V24I | 5′-CATGAAAGATCCGATCATAGTTTCCACCG-3′ | 5′-CGGTGGAAACTATGATCGGATCTTTCATG-3′ |
| PUB23 V29I | 5′-CATGAAAGATCCAATCATAGTCTCTACCG-3′ | 5′-CGGTAGAGACTATGATTGGATCTTTCATG-3′ |
| Construction | ||
| PUB22 | 5′-CTGCAGATGGATCAAGAGATAGA-3′ | 5′-CTGCAGTTAGCCTTTTCTTTAGTC-3′ |
| PUB221-160 | 5′-GAATTCATGGATCAAGAGATAGA-3′ | 5′-GTCGACTCAAAGTGAAGAAGAAGGAGA-3′ |
| PUB22155-435 | 5′-CTGCAGTCTCCTTCTTCTTCACT-3′ | 5′-CTGCAGTTAGCCTTTTCTTTAGTC-3′ |
| PUB23 | 5′-GAATTCATGTCCGGAGGAATAATG-3′ | 5′-GAATTCTCAGCAGGGATATGCAAGAATC-3′ |
| RPN12a | 5′-CTGCAGCAATGGATCCGCAGCTAACT-3′ | 5′-CTGCAGTTACACGATACGCTCCAG-3′ |