Table 1.

Primer List

Gene NameForward Primers (FP)Reverse Primers (RP)
RT-PCR and genotyping PCR
PUB22
    22 FP1 and 22 RP15′-GTTTAGATTCGTTCGTCGTTTC-3′5′-ACTGACACATGTCGTTGAGATTC-3′
    22 FP2 and 22 RP25′-GAATCTCAACGACATGTGTCAGT-3′5′-AAGCAGGATACGAATCATACAAA-3′
    22 FP3 and 22 RP35′-GCTTGAACAACGTACATGTTTG-3′5′-CTACACATAATGATAAACATTGAAAGA-3′
PUB23
    23 FP1 and 23 RP15′-GATCAATATATTCGTCAGGGTTC-3′5′-CCCATTTTCTGAGTCGTTGC-3′
    23 FP2 and 23 RP25′-GCAACGACTCAGAAAATGGG-3′5′-CATCAGCAGGGATATGCAAG-3′
    23 FP3 and 23 RP35′-AACAATACAAACAAGTCCAATTCTC-3′5′-AGCCTGGTAACTTGTGATTTTAT-3′
RD29a5′-CAGGTGAATCAGGAGTTGTT-3′5′-CCGGAAATTTATCCTCTTCT-3′
RD225′-GCAGCGAAGGAGACTCAGCT-3′5′-GTTCCAAGCTGAGGTGTTCTT-3′
18s rRNA5′-GCATTTGCCAAGGATGTTTT-3′5′-GTACAAAGGGCAGGGACGTA-3′
UBC105′-ATGGCGTCGAAGCGGATC-3′5′-TTAGCCCATGGCATACTTCTG-3′
Ub5′-ATGCAGATCTTTGTTAAGACTCTC-3′5′-TCAACCACCACGGAGCCTGA-3′
Site-directed mutagenesis
PUB22 V24I5′-CATGAAAGATCCGATCATAGTTTCCACCG-3′5′-CGGTGGAAACTATGATCGGATCTTTCATG-3′
PUB23 V29I5′-CATGAAAGATCCAATCATAGTCTCTACCG-3′5′-CGGTAGAGACTATGATTGGATCTTTCATG-3′
Construction
PUB225′-CTGCAGATGGATCAAGAGATAGA-3′5′-CTGCAGTTAGCCTTTTCTTTAGTC-3′
PUB221-1605′-GAATTCATGGATCAAGAGATAGA-3′5′-GTCGACTCAAAGTGAAGAAGAAGGAGA-3′
PUB22155-4355′-CTGCAGTCTCCTTCTTCTTCACT-3′5′-CTGCAGTTAGCCTTTTCTTTAGTC-3′
PUB235′-GAATTCATGTCCGGAGGAATAATG-3′5′-GAATTCTCAGCAGGGATATGCAAGAATC-3′
RPN12a5′-CTGCAGCAATGGATCCGCAGCTAACT-3′5′-CTGCAGTTACACGATACGCTCCAG-3′