Table 1.

Isogenic Lines, Alleles, and Tissues from Which the Sequenced Small RNA Populations Were Derived

VarietyaAllelebSeed Coat PhenotypecSource/OriginImmature Seed Tissue Used for Small RNAdTotal ReadsUnique Signatures ≥5 Readse
RichlandIYellowParent line, released 1926Seed coat2,885,86432,870
T157iPigmentedMutant in Richland, 1938Seed coatBlots onlyNA
WilliamsiiYellow, PhParent line, released 1971Seed coat2,886,22227,363
WilliamsiiYellow, PhParent line, released 1971Cotyledon3,033,93127,306
Williams 55iPigmentedMutant in Williams,1973Seed coat6,098,00592,797
  • a Williams is sometimes referred to as Williams 43 or Williams 54, which are internal numbers used in the laboratory, as is Williams 55 to designate the isogenic mutant line. The official designation of the Williams 55 isoline in the USDA germplasm is L885-5495. The T number (T157) of the Richland mutant refers to the official line designation.

  • b All lines are homozygous for the I allele indicated. Dominance relationships are I > ii > i.

  • c Ph, Pigmented hilum in the ii genotype specifies pigment present in the hilum where the seed coat attaches to the pod with an otherwise yellow, nonpigmented seed coat.

  • d Seed coats and cotyledons samples are dissected from midmaturation, green seed at fresh weight range of 50 to 75 mg per seed. NA, not applicable, as no small RNA sequencing was conducted with this line.

  • e The number of unique signatures after adapter trimming that are represented by at least five reads.