Table 1.

FFT-NLLS Analysis of PLHY:luc Expression Patterns in Constant Light

pLHY:luc ConstructPhotoperiod during EntrainmentPeriod (h)AmplitudePhase (h)aSkewnessKurtosisRAEbn
−9574L 20D27.20 ± 0.220.24 ± 0.02−0.70 ± 0.650.29 ± 0.052.71 ± 0.100.29 ± 0.0112
8L16D28.86 ± 0.160.60 ± 0.04−0.73 ± 0.420.24 ± 0.032.69 ± 0.070.32 ± 0.0216
12L12D28.41 ± 0.180.45 ± 0.06−1.69 ± 0.410.21 ± 0.042.48 ± 0.050.34 ± 0.0122
16L8D29.11 ± 0.210.33 ± 0.04+0.49 ± 0.370.08 ± 0.142.04 ± 0.070.37 ± 0.058
20L8D26.16 ± 0.120.25 ± 0.03−5.15 ± 2.99−0.04 ± 0.041.95 ± 0.080.22 ± 0.058
−957 class IA8L16D29.25 ± 0.140.37 ± 0.01###−2.63 ± 0.17##0.14 ± 0.03#2.64 ± 0.050.29 ± 0.0110
−957 class IB8L16D28.95 ± 0.120.33 ± 0.01###−2.93 ± 0.18###0.15 ± 0.03#2.58 ± 0.040.33 ± 0.0112
−957 class IIA8L16D29.12 ± 0.160.25 ± 0.01###−2.44 ± 0.14#0.06 ± 0.05##2.46 ± 0.080.32 ± 0.016
−957 class IIB8L16D28.81 ± 0.110.39 ± 0.03###−0.80 ± 0.520.16 ± 0.02#2.55 ± 0.030.34 ± 0.0118
−957 1,2m8L16D28.91 ± 0.110.41 ± 0.03−1.38 ± 0.540.19 ± 0.022.68 ± 0.040.40 ± 0.0218
12L12D29.58 ± 0.180.40 ± 0.04+0.01 ± 0.60#−0.016 ± 0.09#2.66 ± 0.020.30 ± 0.0112
−957 3,4,5m12L12D28.84 ± 0.100.29 ± 0.03−0.14 ± 1.200.20 ± 0.052.39 ± 0.090.29 ± 0.028
−8474L20D27.22 ± 0.250.18 ± 0.01**−1.38 ± 0.290.09 ± 0.03***2.49 ± 0.05*0.34 ± 0.0217
8L16D28.89 ± 0.250.38 ± 0.01***+1.10 ± 0.36**0.15 ± 0.01**2.56 ± 0.02*0.33 ± 0.0222
12L12D28.26 ± 0.240.32 ± 0.03*−0.97 ± 0.470.07 ± 0.05*2.41 ± 0.060.30 ± 0.0229
16L8D29.50 ± 0.250.21 ± 0.02**+1.11 ± 0.500.03 ± 0.062.46 ± 0.180.37 ± 0.0312
20L8D25.69 ± 0.330.11 ± 0.02***−2.58 ± 2.970.08 ± 0.062.16 ± 0.170.46 ± 0.06***9
−847 3,4,5 m8L16D28.75 ± 0.300.32 ± 0.02#+2.09 ± 0.400.14 ± 0.032.60 ± 0.050.37 ± 0.026
12L12D28.79 ± 0.080.26 ± 0.02+1.26 ± 0.33##0.19 ± 0.062.34 ± 0.030.28 ± 0.0112
16L8D28.19 ± 0.430.22 ± 0.4−1.92 ± 0.80##0.00 ± 0.112.53 ± 0.130.42 ± 0.026
  • FFT-NLLS analysis was carried out between 24 and 130 h in constant light on data that had been normalized to average expression levels in constant light. At least three independent transgenic lines were tested for each construct. Results for each condition were pooled for at least two independent experiments. *, **, and *** indicate P values of 0.05, 0.01, and 0.001 for differences between truncated (−847 PLHY:luc) and full-length (−957 PLHY:luc) constructs. #, ##, and ### indicate P values of 0.05, 0.01, and 0.001 for differences between mutant constructs and the corresponding wild-type construct (−847 or −957 PLHY:luc).

  • a Peak phase relative to dawn. + indicates a phase advance; − indicates a phase delay.

  • b Relative amplitude error (RAE) values are indicative of the quality of the fit to a cosine wave, with RAE values closer to 1 indicative of weaker rhythms.