Table 1.

Nodulation Phenotype of M. truncatula Roots Overexpressing PUB1

Mean No. of Nodules per Plant
DAIExperimentControl3HA-PUB1P Valuea
7 DAIExperiment 16.86 ± 5.81 (n = 96)4.18 ± 4.19 (n = 87)5.27 × 10−4***
Experiment 26.95 ± 5.21 (n = 43)4.29 ± 3.44 (n = 42)7.43 × 10−3**
14 DAIExperiment 19.09 ± 5.96 (n = 96)8.42 ± 5.32 (n = 87)0.42
Experiment 28.8 ± 5.23 (n = 43)7.28 ± 3.99 (n = 42)0.15
  • Transformed wild-type M. truncatula plants overexpressing 3HA-PUB1 or GFP (control) were inoculated with wild-type S. meliloti. Two independent experiments were performed, and the means of the number of nodules formed at 7 and 14 DAI is shown as well as the standard deviations. The number of scored plants is shown in parentheses.

  • a Statistical analysis of nodulation of 3HA-PUB1 versus control plants. P values below 0.01 and 0.001 are marked with two and three asterisks, respectively.