Table 2.

Nodulation Phenotype of PUB1 Knockdown Roots of M. truncatula

Mean No. of Nodules per Plant
M. truncatulaS. melilotiExperimentControlPUB1 RNAiP Valuea
Wild typeWild typeExperiment 110.18 ± 4.77 (n = 44)10.95 ± 5.71 (n = 60)0.47
Experiment 218.16 ± 7.48 (n = 62)17.71 ± 7.89 (n = 63)0.76
Wild typenodLExperiment 13.34 ± 3.43 (n = 44)7.30 ± 4.26 (n = 52)3.90 × 10−6***
Experiment 25.82 ± 3.59 (n = 65)12.15 ± 5.92 (n = 61)3.85 × 10−11***
Wild typenodFnodLExperiment 10.22 ± 0.64 (n = 67)1.53 ± 2.09 (n = 58)3.77 × 10−6***
Experiment 20.17 ± 0.59 (n = 66)2.11 ± 2.14 (n = 65)1.04 × 10−10***
lyk3-4Wild typeExperiment 10.11 ± 0.42 (n = 26)2.79 ± 3.34 (n = 23)1.34 × 10−4***
Experiment 20.28 ± 0.56 (n = 86)3.39 ± 2.99 (n = 90)1.60 × 10−16***
  • Wild-type or lyk3-4 (hcl-4) mutant plants, transformed with the PUB1 RNAi construct or empty vector (control), were inoculated with wild-type or nod mutant bacteria. For each combination, two independent experiments were performed, and the mean number of nodules formed at 21 DAI is shown as well as the standard deviations. The number of scored plants is shown in parentheses.

  • a Statistical analysis of nodulation of PUB1 RNAi versus control plants. P values below 0.001 are marked with asterisks.