Table 3.

Infection Phenotype of PUB1 Knockdown Roots of M. truncatula lyk3-4

Mean No. of ITs per Plant
ExperimentControlPUB1 RNAiP Valuea
Experiment 11.92 ± 3.22 (n = 36)8.88 ± 10.48 (n = 33)3.18 × 10−4***
Experiment 20.48 ± 0.87 (n = 25)6.41 ± 9.54 (n = 22)3.34 × 10−3**
  • lyk3-4 (hcl-4) mutant plants, transformed with the PUB1 RNAi construct or empty vector (control), were inoculated with wild-type bacteria. For each combination, two independent experiments were performed, and the mean number of ITs formed at 5 DAI is shown as well as the standard deviations. The number of scored plants is shown in parentheses.

  • a Statistical analysis of infection of PUB1 RNAi versus control plants. P values below 0.01 and 0.001 are marked with two and three asterisks, respectively.