Table 2.

The Steady State Kinetic Parameters of Recombinant PKSA

SubstrateKm (μM)akcat (10−3 s−1)akcat/Km (M−1 s−1)a
16-OH-C16-CoA25 ± 5.02.8 ± 0.59112
12-OH-C18-CoA23 ± 3.85.0 ± 1.1217
C16-CoA48 ± 3.20.13 ± 0.432.7
  • a Values given are means ± sd; n = 3.