Table 1.

FRET-FLIM Lifetimes of RTNLB Pairs

DonorAcceptorRange of FLIM Lifetimes (ns)Average FLIM Lifetime (ns)
RTNLB1-eGFP2.3–2.42.35
RTNLB1-eGFPmRFP-RTNLB12.1–2.22.15
RTNLB1-eGFPRTNLB1-mRFP2.1–2.22.15
eGFP-RTNLB12.3–2.52.40
eGFP-RTNLB1mRFP-RTNLB12.1–2.22.15
eGFP-RTNLB1RTNLB1-mRFP2.1–2.32.20
RTNLB1 T1-eGFP2.3–2.52.40
RTNLB1 T1-eGFPRTNLB1 T1-mRFP2.1–2.22.15
eGFP-RTNLB132.2–2.52.30
eGFP-RTNLB13mRFP-RTNLB131.9–2.01.95
RTNLB13 T1-eGFP2.3–2.42.35
RTNLB13 T1-eGFPRTNLB13 T1-mRFP2.0–2.22.10
eGFP-RTNLB1mRFP-RTNLB32.1–2.32.20
eGFP-RTNLB1mRFP-RTNLB42.1–2.32.20
eGFP-RTNLB13mRFP-RTNLB32.1–2.22.15
eGFP-RTNLB13mRFP-RTNLB42.0–2.12.05
  • mRFP and eGFP pairs of RTNLB fusions were coexpressed in tobacco epidermal cells and interactions tested through FRET-FLIM. Comparisons with control values without acceptor indicate that all pairs tested interact with one another.