Table 5.

S-Amino Acid Content of Chlamydomonas Proteins

C+M% Weighed by Transcript Abundanceb
List of TranscriptsaC+M % (nwta)b+S (Wild Type)−S (Wild Type)+S (snrk2.1)−S (snrk2.1)+P (Wild Type)−P (Wild Type)
All4.194.554.324.364.544.204.25
−S up (wild type)3.323.832.333.283.094.083.13
−S down (wild type)4.053.933.893.794.243.643.49
−S up (snrk2.1)4.184.254.024.284.284.454.28
−S down (snrk2.1)3.983.663.683.573.643.643.62
−P up (wild type)4.184.423.12c4.734.294.434.06
−P down (wild type)4.184.614.484.374.074.324.30
  • Phosphorous deprivation (−P) was used as internal control. nwta, data not weighted by transcripts abundance.

  • a The list of transcripts that accumulate (up) or decline (down) under the different conditions were selected as described in Figure 3. Subsequently, the genes encoding proteins with no significant homology to a known protein (unknown genes) were filtered out because many of them represent inaccurate v.3.1 gene models and would introduce a strong bias into the data.

  • b S amino acid content percentages are calculated for proteins in the unprocessed state (containing putative signal peptides).

  • c The low values observed under this condition reflects the fact that some transcripts that increase during S deprivation also show a slight increase during –P conditions (D. González-Ballester and A.R. Grossman, unpublished data).