Table 1.

Various Kinetic Constants of the Interaction between GID1 and mSLR1s Using SPR

Binding 1Binding 2
AnalyteLigandka (M−1 s−1)kd (s−1)KD (M) (kd/ka)ka (M−1 s−1)kd (s−1)KD (M) (kd/ka)
GID1SLR1WT2.75 × 1051.62 × 10−35.89 × 10−98.11 × 1056.48 × 10−27.99 × 10−8
GID1HFY315-7AAA2.43 × 1051.30 × 10−35.36 × 10−95.19 × 1054.67 × 10−29.00 × 10−8
GID1PLY321-3AAA2.43 × 1051.13 × 10−34.65 × 10−95.22 × 1055.15 × 10−29.87 × 10−8
GID1HFT327-9AAA2.35 × 1051.95 × 10−38.29 × 10−95.71 × 1055.39 × 10−29.44 × 10−8
GID1QWP356-8AAA1.79 × 1059.64 × 10−45.39 × 10−96.90 × 1056.23 × 10−29.03 × 10−8
GID1HRL450-2AAA1.31 × 1051.06 × 10−38.09 × 10−96.50 × 1056.09 × 10−29.37 × 10−8
GID1SLR1 (E4-R125)3.99 × 1051.31 × 10−23.28 × 10−87.05 × 1055.97 × 10−28.47 × 10−8
GID1SLR1G576V2.78 × 1051.38 × 10−24.96 × 10−86.03 × 1054.38 × 10−27.26 × 10−8
GID1LQ361-2AA1.65 × 1051.48 × 10−28.97 × 10−87.29 × 1056.19 × 10−28.49 × 10−8
GID1DRF490-2AAA4.35 × 1051.01 × 10−22.32 × 10−85.75 × 1055.26 × 10−29.15 × 10−8
GID1HYY497-9AAA5.19 × 1057.01 × 10−31.35 × 10−85.89 × 1055.44 × 10−29.24 × 10−8