Table 2.

Significant Disruption of Fungal Development by RNAi Constructs Targeting Fungal Genes

Clone IDaHIGS ScreenRel. HI (%)bMin.cndPeShort Description (BLASTX)E-Value
HO09I03GP82.3 ± 3.37550.0058Hypothetical (Magnaporthe oryzae)3 × 10−89
HO07E08GP76.0 ± 8.038110.0135Hypothetical (N. crassa)4 × 10−43
HO15J13GP74.9 ± 8.720200.00971,3-β-glucanosyltransferase1 × 10−52
HO11N21GP72.1 ± 7.64770.01061,3-β-glucanosyltransferase1 × 10−82
HO08J20GP71.3 ± 8.55050.0285No match
HO04M11GP71.2 ± 6.55070.0043Hypothetical (G. zeae)5 × 10−18
HO15I23GP65.8 ± 10.23850.0286ADP/ATP carrier protein4 × 10−74
HO14N21GP64.4 ± 8.03880.0030Heat shock protein 701 × 10−133
HO06C14GP59.6 ± 5.93611<0.0001NADH-ubiquinone oxidoreductase1 × 10−111
HO06F11GP59.1 ± 7.62870.0017Vacuolar Ser protease2 × 10−55
HO03F17I mlo582.5 ± 4.47050.0170Endosomal cargo receptor9 × 10−65
HO08A09I mlo579.7 ± 5.06260.0096Hypothetical (Magnaporthe oryzae)1 × 10−22
HO10J21I mlo576.3 ± 6.66350.0228Hypothetical (Magnaporthe oryzae)6 × 10−29
HO12C01I mlo574.9 ± 3.06850.001140S Ribosomal protein6 × 10−32
HO06N16I mlo573.6 ± 9.24350.0458No match
HO10G24I mlo566.4 ± 9.24050.0215No match
  • a Clone ID from the CR-EST database (http://pgrc.ipk-gatersleben.de/cr-est/index.php).

  • b Haustorium index relative to the empty vector control set to 100%.

  • c Min, minimum value (in percent) of rel. HI observed among n independent experiments.

  • d Number of independent experiments.

  • e P value for null hypothesis (no deviation from the empty vector control). The fungal origin of all RNAi target sequences was supported by an EST sequence from in vitro–germinated B. graminis, except for HO06C14 that was instead supported by a highly similar sequence in the draft genomic sequence of B. graminis.