Table 1.

SPN Formation and Complementation of ccamk Mutants by GOF-CCaMK

Spontaneous Nodule FormationaSymbiont Entry into ccamk Mutantsa
CCaMK VariantWild TypeccamkRhizobiaMycorrhiza
Vector control0% (0/64)0% (0/35)0% (0/16)0%(0/55)
CCaMKTT (wild type)0% (0/40)0% (0/45)86% (32/37)79% (27/34)
CCaMKTD87% (41/47)41%(14/34)75% (15/20)64% (18/28)
CCaMK31416% (5/32)32%(11/34)0% (0/14)29% (9/31)
CCaMK314-NLS39% (9/23)26% (6/23)0% (0/27)17% (5/30)
CCaMK314TD17% (5/29)33% (9/27)0% (0/24)18% (9/50)
CCaMK314TD-NLS15% (3/19)15% (6/39)0% (0/25)57% (16/28)
CCaMK314TD-NES2% (1/48)2% (1/53)0% (0/35)2% (1/43)
  • Values in parentheses indicate numbers of SPN formed or complemented roots/total transformed roots.

  • a SPN formation was observed at 4 weeks after transferring to a sterilized soil. Infection of rhizobia or arbuscular mycorrhiza was observed at 4 and 3 weeks after inoculation, respectively.