Table 1.

Kinetic Parameters of L/ODCs and La-L/ODC Mutant Proteins

ProteinKm (mM)Vmax (nmol min−1μg−1kcat (s−1)kcat/Km (M−1 s−1)LDC/ODC Ratio of kcat/Km
LDCODCLDCODCLDCODCLDCODC
La-L/ODC_wild type2.371.051.491.141.1800.914338590.500
Sf-L/ODC2.101.532.941.462.3201.1611087551.470
Ek-L/ODC3.851.322.420.761.9100.734694541.030
Ng-ODCa1.590.561.52 × 10−41.25 × 10−20.0070.584.410350.004
La-L/ODC-M341L2.491.044.900.373.8800.2915582795.580
La-L/ODC-F344Y5.261.597.990.516.3300.4012032534.750
La-L/ODC-F344H97.841.53181.822.90143.9802.29147114960.980
  • All experiments were performed in 50 mM potassium phosphate buffer, pH 7.5. Kinetic parameters were calculated from mean values (n = 3 to 4).

  • a Recalculated from reported values for N. glutinosa L/ODC (Lee and Cho, 2001).