Table 1.

Substrate Specificity of AAE3

SubstrateStructureActivity
μmoles/min/mg Protein
OxalateHOOC-COOH5.8
MalonateHOOC-CH2-COOH<0.1
SuccinateHOOC-(CH2)2-COOH<0.1
MalateHOOC-CH2 –CHOH-COOH<0.1
AcetateCH3-COOH<0.1
FormateH-COOH<0.1
LactateCH3-CHOH-COOH<0.1
GlycolateCHOH-COOH<0.1
GlyoxylateCHO-COOH<0.1
GlutarateHOOC(CH2)3-COOH<0.1
  • CoA synthetase activities were determined at pH 7.5, with a substrate concentration of 300 μM.