Table 4. Redistribution of Heavy Label following Glc Feeding of Tomato OGDH Antisense Lines and Wild-Type Leaves
MetabolitesWild TypeOGDH14OGDH36OGDH37
μmol C1 equivalent g−1 fresh wt h−1
Ala4.8687 ± 1.25594.0151 ± 0.58194.4456 ± 0.71164.4152 ± 0.6440
Asn0.1591 ± 0.04490.1250 ± 0.00460.1319 ± 0.01450.1655 ± 0.0160
Asp0.1355 ± 0.02130.0636 ± 0.01520.0641± 0.01560.0723 ± 0.0131
Fru0.5056 ± 0.03390.4099 ± 0.11320.6478 ± 0.07860.7956 ± 0.1236
Fumarate0.1308 ± 0.06850.1143 ± 0.01280.1627 ± 0.06620.1367 ± 0.0334
GABA0.0770 ± 0.00610.1161 ± 0.00630.9772 ± 0.00740.1021 ± 0.0157
Glu1.2066 ± 0.17311.7232 ± 0.16401.6014 ± 0.13461.9555 ± 0.2489
Gln0.9369 ± 0.13490.9130 ± 0.09561.1067 ± 0.20011.4611 ± 0.2310
Gly0.7898 ± 0.1448ndndnd
His1.0570 ± 0.14820.9155 ± 0.04790.9450 ± 0.12520.9976 ± 0.2123
Ile0.0190 ± 0.00480.0164 ± 0.00320.0126 ± 0.00300.0318 ± 0.0090
Lys1.5608 ± 0.23361.4119 ± 0.15091.4434 ± 0.19611.4965 ± 0.4709
Malate2.1743 ± 0.16581.9227 ± 0.15831.8409 ± 0.20562.0265 ± 0.3760
Met0.0437 ± 0.00130.0068 ± 0.00060.0023 ± 0.00110.0139 ± 0.0045
Orn0.3665 ± 0.10850.0736 ± 0.01550.0633 ± 0.02020.1186 ± 0.0454
2-Oxoglutarate0.3223 ± 0.16871.1038 ± 0.03490.8570 ± 0.12640.8754 ± 0.0837
Phe0.0600 ± 0.01220.0464 ± 0.01130.0722 ± 0.01070.0850 ± 0.0136
Pro0.0308 ± 0.01240.0011 ± 0.00030.0017 ± 0.00100.0044 ± 0.0026
Ser0.1215 ± 0.02350.0918 ± 0.01440.0926 ± 0.01860.1032 ± 0.0330
Succinate1.3544 ± 0.39763.4968 ± 0.40903.0023 ± 0.33753.2060 ± 0.3398
Suc1.3267 ± 0.13471.1869 ± 0.32731.1997 ± 0.25621.1500 ± 0.3886
Trp0.5457 ± 0.09130.7438 ± 0.06430.5941 ± 0.22600.6641 ± 0.0481
Val0.4520 ± 0.10980.3271 ± 0.05430.4440 ± 0.06170.4246 ± 0.0398
  • Fully expanded leaves of 4-week-old plants were harvested at the middle of the light period and fed via the petiole with [U-13C]Glc solution. Values represent absolute redistribution of the label and are given as means ± se of determinations on six independent plants. Those in boldface were determined by Student’s t test to be significantly different (P ≤ 0.05) from the wild-type. nd, not determined.