Table 3. Enzyme Activities in Leaves of OGDH Transgenic Tomato Lines
EnzymesOGDH36OGDH37ParameterOGDH36
Phosphoenolpyruvate carboxylase253.3 ± 11.5258.5 ± 24.2308.4 ± 15.2268.4 ± 17.5
AGPase1598.5 ± 265.11662.4 ± 217.81478.9 ± 210.61423.5 ± 127.6
Transketolase208.9 ± 12.1215.8 ± 12.1227.9 ± 15.7234.0 ± 12.4
Transaldolase21.2 ± 2.424.8 ± 1.624.2 ± 1.824.9 ± 2.0
FBPase178.6 ± 39.7203.7 ± 27.9191.4 ± 53.2180.8 ± 52.1
NADP-glyceraldehyde 3-phosphate dehydrogenase70.9 ± 5.756.0 ± 4.458.2 ± 2.265.2 ± 3.1
Aconitase34.2 ± 8.736.4 ± 2.335.5 ± 4.730.6 ± 3.9
Succinyl-CoA ligase13.1 ± 0.913.6 ± 1.513.3 ± 2.215.8 ± 4.0
Fumarase3431.1 ± 256.123.1 ± 226.73585.5 ± 192.93223.1 ± 226.7
NAD-malate dehydrogenase56.4 ± 4.256.2 ± 5.465.6 ± 2.855.9 ± 4.5
NADP-malate dehydrogenase initial90.3 ± 4.2103.5 ± 7.9105.4 ± 6.5102.3 ± 8.6
NADP-malate dehydrogenase total234.7 ± 8.1223.6 ± 22.9241.2 ± 12.2240.4 ± 1.3
NADP-malate dehydrogenase activation state (%)38.5 ± 4.247.3 ± 6.243.8 ± 5.342.7 ± 2.6
NADP-isocitrate dehydrogenase1572.9 ± 154.21615.0 ± 247.41493.2 ± 247.31425.7 ± 217.9
NAD-isocitrate dehydrogenase29.2 ± 1.326.6 ± 1.927.1 ± 2.927.9 ± 1.6
Pyruvate dehydrogenase9.6 ± 2.211.1 ± 1.511.3 ± 1.510.8 ± 0.9
Ferredoxin-dependent glutamate synthase3697.4 ± 423.33261.5 ± 338.63399.1 ± 431.93257.3 ± 351.7
Nitrate reductase352.7 ± 30.4316.7 ± 37.6307.6 ± 69.7328.3 ± 33.4
Glu dehydrogenase270.5 ± 24.5258.5 ± 24.2257.3 ± 15.2248.4 ± 17.5
  • NAD-malate dehydrogenase activity is expressed in mmol min−1 g−1 fresh weight.

  • Activities were determined in 4-week-old fully expanded source leaves harvested 6 h into the photoperiod. Data presented are means ± se (n = 6). Values in boldface were determined by Student’s t test to be significantly different (P < 0.05) from the wild type.