Table 2. Cell Wall Composition and Digestion Efficiency Analysis of MOMT4 Transgenic Plants
LinesTotal Acetyl Bromide Lignin (mg/g CWR)Lignin CompositionCellulose
(mg/g CWR)Digestion Efficiency
(Weight Loss %)
Monomers from β-O-4–Linked H, G, and S Conventional Lignin Units (µmol/g CWR)aMonomers from β-O-4–Linked H, G, and S Conventional Lignin Units (µmol/g lignin)bS/G
HGSHGS
Control 1152.37 ± 7.9NDc145.31 ± 8.5184.41 ± 5.53ND1008.35 ± 54.95583.27 ± 27.370.58591.18 ± 54.739.8 ± 0.82
Control 2154.71 ± 10.85ND140.90 ± 2.6276.07 ± 2.62ND931.22 ± 69.55505.20 ± 64.280.54580.97 ± 3.2339.97 ± 3.90
Control 3143.64 ± 5.43ND140.05 ± 18.8972.56 ± 11.99ND898.22 ± 85.73464.94 ± 58.620.52553.90 ± 28.2239.07 ± 2.25
MOMT4-1128.81 ± 3.25**ND123.54 ± 5.92*59.15 ± 3.89**ND939.62 ± 46.22449.66 ± 28.980.48582.57 ± 26.7844.05 ± 0.36*
MOMT4-2130.63 ± 2.32*ND118.57 ± 4.80**64.29 ± 2.64**ND879.73 ± 48.29476.93 ± 25.880.54532.12 ± 8.9944.9 ± 1.76*
MOMT4-3113.47 ± 1.90 **ND92.36 ± 7.82**51.46 ± 4.95**ND803.04 ± 76.55447.35 ± 46.780.56570.10 ± 8.2847.62 ± 1.42**
MOMT4-4126.62 ± 2.2 **ND113.30 ± 4.29**60.37 ± 2.42**ND898.08 ± 51.02478.48 ± 29.560.53582.89 ± 14.1142.99 ± 1.28
  • The data represent the means and se from triplicate or more biological analyses (n ≥ 3), except the data for lignin monomer and cellulolysis of control plants are from duplicate analyses. Asterisks indicate significant difference compared to the average value of control lines from three independent transgenic events: *Student’s t test, P < 0.05; **Student’s t test, P < 0.01.

  • a Yield in main lignin-derived thioacidolysis monomers (expressed in micromoles per gram of extract-free cell wall residues [CWR]) recovered from conventional H, G, or S β-O-4–linked lignin units.

  • b The monomers of b were expressed in micromoles per gram acetyl bromide lignin.

  • c ND, not detectable.