Table 2. Effect of Silencing of NCED4 on Lettuce Seed Set and Seed Weight
LineSeeds per FlowerWeight per SeedUndeveloped Seeds per Flower
RNAi2-CTL10.93 ± 0.791.11 ± 0.016.12 ± 0.29
RNAi8-CTL12.20 ± 0.791.32 ± 0.026.05 ± 0.36
RNAi8-76.57 ± 0.35**1.55 ± 0.02**6.10 ± 0.28
RNAi8-46.40 ± 0.38**1.54 ± 0.03**6.15 ± 0.29
  • For seed set, seeds (full and undeveloped) of 15 buds for each replicate were collected for four replicates each from two homozygous RNAi lines (RNAi8-4 and RNAi8-7) and two RNAi control lines (RNAi2-CTL and RNAi8-CTL). For seed weight, 50 mature seeds were weighed for each replicate for each line. Statistical analysis by a one-tailed Student’s t test indicated significant differences (**P < 0.01).