Table 3. Effect of Polar Auxin Transport Inhibitors on Wild-Type, zifl1-Mutant, and ZIFL1-Overexpressing Seedlings
GenotypeRelative PR elongation (%)a
Control10 µM NOA10 µM NPA1 µM TIBA
Col-0100.00 ± 5.8976.06 ± 6.2738.09 ± 9.8475.67 ± 9.91
zifl1-1100.00 ± 4.7674.28 ± 8.18 (0.26)59.00 ± 6.57 (6.0e−8)90.19 ± 9.82 (1.7e−4)
zifl1-2100.00 ± 6.7973.02 ± 6.36 (0.10)55.02 ± 9.98 (2.6e−5)87.65 ± 4.82 (1.1e−4)
ZIFL1.1OX100.00 ± 3.6073.40 ± 6.97 (0.15)24.08 ± 3.60 (7.5e−6)57.15 ± 7.55 (1.3e−6)
ZIFL1.3OX100.00 ± 6.0674.53 ± 6.90 (0.27)38.04 ± 6.43 (0.49)78.49 ± 8.63 (0.21)
  • Numbers between parentheses indicate the P values (the wild type versus zifl1 mutants or ZIFL1-overexpressing lines) obtained by Student’s t test.

  • a Effect of the auxin polar transport inhibitors NOA, NPA, and TIBA on PR elongation of 12-d-old seedlings of the wild-type (Col-0), the zifl1 mutants (zifl1-1 and zifl1-2), and the ZIFL1.1OX or ZIFL1.3OX transgenic line. Results are representative of three independent experiments, and values represent means ± sd (n = 16).