Table 1. Complementation of zifl1 Mutant Phenotypes by the ZIFL1.1 and ZIFL1.3 Isoforms
GenotypePR Elongation (cm)aWater Loss (% FW)b
Control0.025 µM 2,4-D0.05 µM IAA
Col-04.56 ± 0.462.93 ± 0.333.98 ± 0.6620.53 ± 1.65
zifl1-14.63 ± 0.532.41 ± 0.26 (1.8e−5)3.05 ± 0.42 (3.7e−5)35.86 ± 1.53 (1.12e−5)
zifl1-24.57 ± 0.692.38 ± 0.47 (3.9e−4)2.95 ± 0.64 (8.2e−5)36.44 ± 4.44 (5.6e−4)
zifl1-1/ProZIFL1:ZIFL1.1-GFP4.39 ± 0.682.77 ± 0.62 (0.20)3.65 ± 0.65 (0.10)34.73 ± 1.67 (2.2e−5)
zifl1-1/ProZIFL1:ZIFL1.3-GFP4.49 ± 0.412.46 ± 0.37 (4.2e−4)2.97 ± 0.47 (1.9e−5)20.89 ± 3.79 (0.44)
zifl1-2/ProZIFL1:ZIFL1.1-GFP4.34 ± 0.462.76 ± 0.33 (0.09)3.82 ± 0.32 (0.21)36.24 ± 2.29 (3.6e−5)
zifl1-2/ProZIFL1:ZIFL1.3-GFP4.42 ± 0.392.39 ± 0.41 (1.7e−4)2.80 ± 0.43 (1.3e−6)21.32 ± 3.15 (0.36)
  • Numbers between parentheses indicate the P values (wild type versus zifl1 mutants or ZIFL1 transgenic lines) obtained by Student’s t test.

  • a Effect of 2,4-D and IAA on PR elongation of 12-d-old seedlings of the wild type (Col-0), the zifl1 mutants (zifl1-1 and zifl1-2), and two independent mutant lines complemented with either the ProZIFL1:ZIFL1.1-GFP or ProZIFL1:ZIFL1.3-GFP construct. Results are representative of three independent experiments, and values represent means ± sd (n = 16).

  • b Water loss rates of 5-week-old irrigated plants of the wild type (Col-0), the zifl1 mutants (zifl1-1 and zifl1-2), and two independent mutant lines complemented with either ProZIFL1:ZIFL1.1-GFP or ProZIFL1:ZIFL1.3-GFP. Results are representative of three independent experiments, and values represent means ± sd (n = 4). FW, fresh weight.