Table 2. Accumulation of Radiolabeled Auxins in Yeast and Arabidopsis
GenotypeRelative Auxin Accumulation
2,4-DIAANAA
Yeasta
Δtpo1 + pGREG57613.97 ± 1.2928.70 ± 2.08ND
Δtpo1 + GFP-ZIFL1.17.26 ± 0.89 (1.2e−6)12.96 ± 2.36 (2.1e−7)ND
Δtpo1 + GFP-ZIFL1.37.40 ± 0.64 (6.7e−7)15.03 ± 1.70 (2.4e−7)ND
Arabidopsisb
Col-0100.00 ± 11.64100.00 ± 11.99100.00 ± 14.38
zifl1-1141.70 ± 10.40 (3.8e−11)120.52 ± 9.26 (1.6e−6)103.94 ± 14.91 (0.22)
zifl1-2134.88 ± 19.89 (6.5e−6)121.83 ± 12.41 (1.4e−5)106.19 ± 16.16 (0.13)
ZIFL1.1OX77.38 ± 8.43 (1.2e−7)85.71 ± 9.19 (1.3e−4)99.53 ± 9.87 (0.45)
ZIFL1.3OX96.74 ± 16.00 (0.27)95.24 ± 11.74 (0.12)106.98 ± 17.67 (0.11)
  • Numbers between parentheses indicate the P values (yeast Δtpo1 mutant versus ZIFL1.1 or ZIFL1.3-expressing Δtpo1 lines, or Arabidopsis wild type versus zifl1 mutants or ZIFL1-overexpressing lines) obtained by Student’s t test. ND, not determined.

  • a Accumulation ratio (intra/extracellular) of [14C]2,4-D or [14C]IAA in nonadapted yeast Δtpo1 mutant cells harboring either the cloning vector pGREG576 or the pGREG576_ZIFL1.1 or pGREG576_ZIFL1.3 plasmids. Results are from three independent experiments, and values represent means ± sd (n = 6).

  • b Relative [14C]2,4-D, [14C]IAA, or [14C]NAA accumulation in root tips from 5-d-old seedlings of the wild type (Col-0), the zifl1 mutants (zifl1-1 and zifl1-2), and the ZIFL1.1OX or ZIFL1.3OX transgenic lines. Data are expressed relative to wild-type accumulation. Results are from three independent experiments, and values represent means ± sd (n = 12).