Table 1. Examples of Pathways with Ubiquitylation Targetsa
NameGene LocusFunction/ActivityMG132b
RNA Silencing
 AGO1At2g48410RNA splicer+
 AGO2cAt1g31280RNA splicer+
 AGO10/PNHAt5g43810RNA splicer
 HEN2At2g06990RNA helicase+
 SEAt2g27100RNA splicing+
 VCSAt3g13300mRNA decapping+
ABA Signaling
 PYR1At4g17870Abscisic acid receptor+
 SnRK1.1At3g01090Protein kinase+
 SnRK2.4At1g10940Protein kinase+
 OST1At4g33950Protein kinase+
 SUAAt3g54230splicing factor+
 MPK3At3g45640MAP kinase+
 MPK4At4g01370MAP kinase+
 CDPK2 (CPK11)At1g35670Protein kinase+
 CDPK6 (CPK3)At4g23650Protein kinase+
 RCN1At1g25490Protein phosphatase+
Auxin Signaling
 ABF4cAt4g24390FBX auxin receptor
 BIG/TIR3At3g02260Auxin transport+
 ABCB4At2g47000Auxin efflux+
 CAND1At2g02560CRL regulator+
 CSN5AAt1g22920CSN CRL regulator+
 CSN6AAt5g56280CSN CRL regulator+
 IBR5At2g04550Protein phosphatase
 PP2AAt1g69960Protein phosphatase+
 RCN1At1g25490Protein phosphatase+
 PP2A-A2At3g25800Protein phosphatase+
 PP2A-A3At1g13320Protein phosphatase+
BR Signaling+
 ST1At2g03760BR sulfotransferase+
 ST4AAt2g14920BR sulfotransferase+
 BSK1At4g35230Protein kinase+
 RCN1At1g25490Protein phosphatase+
 PP2A-A2At3g25800Protein kinase+
 PP2A-A3At1g13320Protein kinase+
 GRF10At1g2230014-3-3 Protein
Circadian Rhythms
 PRR7cAt5g02810Pseudoresponse regulator+
 CKA1At5g67380Casein kinase+
 CKA2At3g50000Casein kinase+
 EPR1At1g18330Early Phy response transcription factor
Nitrate Metabolism
 NR2At1g37130Nitrate reductase+
 MPK3At3g45640MAP kinase+
 CDPK6 (CPK3)At4g23650Protein kinase+
 GRF1At4g0900014-3-3 Protein
 GRF2At1g7830014-3-3 Protein
 GRF3At5g3848014-3-3 Protein+
 GRF6At5g1045014-3-3 Protein
 GRF8At5g6543014-3-3 Protein+
Phenylpropanoid Metabolism
 PAL1At2g37040Phe ammonia lyase
 PAL2At3g53260Phe ammonia lyase
 PAL3At5g04230Phe ammonia lyase
 C4HAt2g30490Cinnamate-4-hydroxylase
 HCTAt5g48930HC-CoA transferase+
 CADAt1g72680Cinnamyl alcohol dehydrogenase
 COMTAt5g54160Caffeic methyltransfer+
Innate Immunity
 PCS1At5g44070Phytochelatin synthase
 PEN2At2g44490β-Glucosidase+
 PEN3At1g59870ABC transporter
 PAD2At4g23100Glu-Cys ligase+
  • a See Supplemental Data Set 2 online for the complete list.

  • b Plus and minus signs identify ubiquitylated proteins whose PSM abundance either increased (+) or remained the same or decreased (−) upon treating the plants with MG132.

  • c One or more Ub-binding sites mapped (see Supplemental Data Set 5 online).