Table 1. List of Genetically Encoded Ratiometric pH-Sensitive Constructs Used in This Study
Subcellular CompartmentpH Sensor DescriptionConstruct Name
Cytoplasm; nucleuspHluorinCyto-pH
ERTobacco chitinase SP, pHluorin, HDEL (1)pH-HDEL
trans-GolgiSialyltransferase, pHluorin (2 and 3)ST-pH
TGN and early endosomesAt-VSR2;1 SP, pHluorin, At-VSR2;1-YA (4 to 7)pH-VSR-Y
Prevacuole and TGNAt-VSR2;1 SP, pHluorin, At-VSR2:1 (5 and 8 to 10)pH-VSR
Late prevacuoleAt-VSR2;1 SP, pHluorin, At-VSR2:1-IMAA (5)pH-VSR-IM
Acidic/lytic vacuole and late prevacuolePetunia (Petunia hybrida) aleurain SP, vacuolar sorting determinant of petunia aleurain, pHluorin (11 and 12)Aleu-pH
  • References in the second column are as follows: 1, Gomord et al. (1997); 2, Wee et al. (1998); 3, Boevink et al. (1998); 4, daSilva et al. (2006); 5, Saint-Jean et al. (2010); 6, Foresti et al. (2010); 7, Drakakaki et al. (2006); 8, Paris et al. (1997); 9, Ahmed et al. (1997); 10, Li et al. (2002); 11, Di Sansebastiano et al. (2001); 12, Humair et al. (2001).