Table 1. Monosaccharide Composition of Wild-Type and PGX1 Mutant Cell Walls
TissueGenotypeFucRhamnoseAraGalGlcXylGalAGlcA
ESCol5.74 ± 0.425.37 ± 0.9035.33 ± 3.1016.38 ± 1.025.55 ± 0.918.61 ± 0.5923.03 ± 2.983.73 ± 0.59
PGX1AT6.81 ± 0.755.81 ± 0.6432.80 ± 1.3516.96 ± 1.096.01 ± 0.608.49 ± 0.6622.02 ± 2.673.34 ± 0.98
PGX1OE-16.88 ± 0.555.72 ± 0.4534.69 ± 3.2218.15 ± 1.115.10 ± 0.628.42 ± 0.2219.29 ± 2.283.51 ± 0.70
PGX1OE-487.14 ± 0.425.27 ± 0.6530.76 ± 2.0521.33 ± 2.745.92 ± 0.698.80 ± 0.7019.07 ± 2.723.40 ± 0.66
pgx1-16.33 ± 0.554.69 ± 0.5933.97 ± 1.8716.60 ± 1.075.60 ± 0.768.99 ± 0.6723.82 ± 4.070.54 ± 0.20
pgx1-26.56 ± 0.595.77 ± 1.3830.39 ± 1.7919.69 ± 1.386.10 ± 0.217.83 ± 0.3922.36 ± 3.162.60 ± 0.93
LCol2.84 ± 0.561.93 ± 0.158.01 ± 0.502.67 ± 0.1150.10 ± 1.514.00 ± 0.2030.41 ± 2.05
PGX1AT2.77 ± 0.321.43 ± 0.065.94 ± 0.251.97 ± 0.1253.90 ± 3.713.23 ± 0.0530.79 ± 3.90
PGX1OE-12.50 ± 0.771.53 ± 0.115.57 ± 0.232.32 ± 0.1867.80 ± 1.004.16 ± 0.4216.14 ± 1.40
PGX1OE-482.93 ± 0.211.66 ± 0.046.04 ± 0.202.58 ± 0.1461.10 ± 1.493.38 ± 0.1022.33 ± 1.78
pgx1-12.24 ± 0.221.16 ± 0.054.84 ± 0.391.46 ± 0.0864.10 ± 1.743.39 ± 0.1722.83 ± 1.87
pgx1-22.10 ± 0.291.26 ± 0.085.01 ± 0.441.57 ± 0.1459.00 ± 6.633.33 ± 0.1027.76 ± 7.35
  • Sugar composition (GalA, galacturonic acid; GlcA, glucuronic acid) from 6-d-old etiolated seedlings (ES) and leaves from 40-d-old plants (L) of the wild type, PGX1AT, PGX1OE-1, PGX1OE-48, pgx1-1, and pgx1-2 genotypes. Sugar amounts are presented as mean percentages ± sd (n = 6). Bold values indicate mutant glycosyl residue compositions that are at least ±15% and are significantly different (P < 0.05, t test) compared to the wild type for at least two overexpression and/or for both mutant lines.