Table 1. Summary of Mutations and Coverage Obtained for the EMS-Mutagenized Individuals in Each Capture Reaction
Capture Name2345Wheat
EMS201014286
EMS seed contaminant00020
No read obtained00020
Control00011
Genotype00800
Total201022327
Percentage of base pairs on target64.5 ± 2.372.6 ± 0.657.3 ± 0.424.1 ± 0.649.1 ± 1.2
Mean no. of reads (million)12.1 ± 7.816.2 ± 3.03.9 ± 1.311.2 ± 3.133.0 ± 5.2
Sequencing lanesa110.511
Fold increase coverage on versus off targeted regions15.8 ± 1.622.9 ± 0.711.6 ± 0.22.7 ± 0.1n/a
Mean no. of mutations/individual246 ± 176316 ± 37870 ± 59508 ± 3611178 ± 187b
Mean no. of het. mut./Mb3.9 ± 2.94.6 ± 5.15.0 ± 4.15.8 ± 3.516.9
Mean no. of hom. mut./Mb2.0 ± 0.82.5 ± 2.81.7 ± 0.93.1 ± 1.83.2
Overall mutation rate (no. of mut./Mb)5.9 ± 3.47.0 ± 7.86.7 ± 4.58.9 ± 5.220.1
Percentage of deleterious mutations18.4 ± 2.519.4 ± 3.621.3 ± 6.211.7 ± 3.0n/a
  • All statistics are calculated per sample and averaged per capture reaction. Mean standard deviations are indicated. Control nonmutagenized samples, genotypes, and individuals suspected to be seed contaminants are not included in the statistics (see Methods). n/a, not available.

  • a 100 PE HiSeq 2000 sequencing lane.

  • b The average number of mutations per wheat lines was calculated at a minimum mutant allele coverage of 4 for heterozygous mutations and a minimum mutant allele coverage of 7 for heterozygous mutations (adjusted for 3% false positive rate, based on the Kronos control sample).