Table 2. Kinetic Properties of Recombinant At4g19010
Km (μM)kcat (s−1)kcat/Km (s−1⋅mM−1)
t-Cinnamate112 ± 370.7 ± 0.26 ± 2
p-Coumarate125 ± 252.1 ± 0.417 ± 3.4
Ferulate113 ± 453.6 ± 1.432 ± 13
Caffeate85 ± 122.4 ± 0.328 ± 4
Sinapaten.d.n.d.n.d.
4-Hydroxybenzoate67 ± 300.01 ± 0.010.15 ± 0.07
Acetaten.d.n.d.n.d.
  • Data are means of six experimental replicates from two enzyme purifications ± se. n.d., not determined, for no activity was detected at any of the substrate concentrations tested.