Table 2. Kinetic Analysis of PDT and ADT Activity of the C. tepidum ADT Homolog
Substratekcat (s−1)Km (μM)kcat/Km (mM−1 s−1)
Arogenate5.4 ± 0.21544 ± 1223.5 ± 0.2
Prephenate0.27 ± 0.04827 ± 870.32 ± 0.02
  • Kinetic parameters were obtained for arogenate (ADT activity) and prephenate (PDT activity) in 5- and 30-min reactions using 2 and 37.2 μg/mL recombinant enzyme, respectively. Data are means ± se (n = 3 independent experiments).