Table 1. Kinetic Analysis of C. tepidum and Synechocystis sp PCC6803 PPA-AT Homologs
Substratekcat (s−1)Km (μM)kcat/Km (mM−1 s−1)
C. tepidum PPA-AT homolog
 Prephenate12.3 ± 0.2464 ± 3926.8 ± 2.5
 α-Ketoglutarate17.2 ± 1.9949 ± 10218.9 ± 3.9
Synechocystis sp PCC6803 PPA-AT homolog
 Prephenate1.1 ± 0.12539 ± 2540.44 ± 0.02
 α-Ketoglutarate54.2 ± 3.7486 ± 33111.7 ± 5.7
  • Kinetic parameters were obtained for prephenate and α-ketoglutarate in 5-min reactions using 0.5 and 2 μg/mL recombinant enzymes, respectively, for C. tepidum PPA-AT homolog and 8 and 0.5 μg/mL for Synechocystis homolog. Data are means ± se (n = 3 independent experiments). 20 mM aspartate and 200 μM PLP were used as an amino donor and a cofactor, respectively.