Table 5. Kinetic Constants of Mt-CAD2 Mutants with Sinapaldehyde as Substrate
Mt-CAD2 ProteinKm (μM)Vmax (nmol mg−1 Protein s−1)kcat (s−1)kcat/Km (μM−1⋅s−1)
Wild type730.00.640.02432.8
Y136F279.21.020.039139.7
F226A282.61.220.044155.7
Y136F/F226A234.72.180.083353.6
S130ANDAa
Y165ANDA
Y165FNDA
K169ANDA
  • a NDA, no detectable activity.