Table 3. Effect of Added Ligands on Melting Temperature of Petunia CCR1 and M. truncatula CCR1, CAD1, and CAD2 Proteins
Tm (in °C) in the Presence of Added Ligand
Protein (10 μM)NoneNADP+ (500 μM)CoA (1 mM)NADP+ (500 μM) + CoA (1 mM)Taxifolin (200 μM) + NADP+ (500 μM)
Ph-CCR133434549ND
Mt-CCR235424752ND
Mt-CAD15264566266
Mt-CAD25262616064.5
  • ND, not determined.