Table 2. Kinetic Constants of Mt-CAD1 and Mt-CAD2 with Various Hydroxycinnamaldehyde Substrates
Kinetic ParameterCoumaraldehydeConiferaldehydeSinapaldehyde
CAD1CAD2CAD1CAD2CAD1CAD2
Km (μM)8.09.408.56.6010.9730.0000
Vmax (nmol mg−1 protein s−1)402.0119.00229.027.30260.00.6400
kcat (s−1)15.60.469.10.1010.20.0240
kcat/Km (mM−1⋅s−1)1950.048.901070.015.30926.00.0328