Table 1. Kinetic Parameters of AtCPT7 with GPP, FPP, or GGPP as Substrate
SubstrateKm (µM)Kcat (s−1)Kcat/Km (s−1 M−1)
GPP43.91 ± 11.350.106 ± 0.0082.41 × 103
FPP3.83 ± 0.720.104 ± 0.0042.70 × 104
GGPP5.87 ± 0.910.109 ± 0.0041.85 × 104
  • Measurements were made at room temperature in 50 mM HEPES buffer, pH 8.0, containing, 5 mM DTT, 7.5 mM MgCl2, 100 mM KCl, 10% (v/v) glycerol, and 0.1% (v/v) Triton X-100. Reactions were started by adding 14C-IPP substrate. Data are the means of three independent determinations ± sd.