Table 1. Analysis of Currents Recorded in Whole-Cell Configuration from Wild-Type and Mutant KAT1 Channels
V1/2SCurrent Densityn
KAT1 WT−99.5 ± 0.5 mV13.5 ± 0.532.1 ± 4.2 pA/pF65
KAT1 WT + FC−82.8 ± 0.5 mV13.9 ± 0.660 ± 1.9 pA/pF31
KAT1 S676A−110 ± 0.7 mV13.1 ± 0.518.2 ± 2.3 pA/pF25
KAT1 S676A + FC−112.4 ± 0.9 mV12.8 ± 0.418.9 ± 2.5 pA/pF8
KAT1 S676D−84.9 ± 0.4 mV12.3 ± 0.472 ± 7 pA/pF13
KAT1 S520A−100.1 ± 0.4 mV12.2 ± 0.333.6 ± 4.9 pA/pF11
KAT1 S578A−101.1 ± 0.5 mV13.3 ± 0.530.5 ± 4 pA/pF10
KAT1 S520A/S578A−99.5 ± 0.3 mV14.4 ± 0.329.1 ± 5.1 pA/pF10
KAT1 F674A−99.1 ± 0.4 mV13.6 ± 0.433 ± 5 pA/pF10
KAT1 WT + CPP−112 ± 0.8 mV13.5 ± 0.616 ± 1.5 pA/pF10
KAT1 P678−100 ± 0.9 mV14.6 ± 0.831.6 ± 1.4 pA/pF8
KAT1 P678 + FC−84.6 ± 0.8 mV15 ± 0.867 ± 1.8 pA/pF9
KAT1 ENS680−100.5 ± 0.5 mV14.4 ± 0.536.5 ± 0.5 pA/pF8
KAT1 ENS680 + FC−86.7 ± 0.4 mV13.4 ± 0.466 ± 2 pA/pF8
KAT1 N677V−102.6 ± 0.4 mV13.7 ± 0.330.9 ± 3.9 pA/pF8
KAT1 N677V + FC−83.1 ± 0.6 mV14 ± 0.560.9 ± 2.3 pA/pF8
KAT1 N677V/P680−100.4 ± 0.9 mV14.9 ± 0.832.6 ± 4.2 pA/pF8
KAT1 N677V/P680+ FC−100.9 ± 0.7 mV15.3 ± 0.728.8 ± 2.3 pA/pF8
  • V1/2 and S are the parameters obtained by the Boltzmann fit of the activation curve and correspond to the half activation voltage and the inverse slope factor, respectively. Current density was calculated by dividing the current value recorded at –135 mV for the cell capacitance. n is the number of cells. WT, wild type.