Parameter of Gating and Current Kinetics | ALMT9 (n = 7) | ALMT4 Wild Type (n = 8) | ALMT4 S382A (n = 8) |
---|---|---|---|

I max (pA)_{tail0} | 121 ± 38^{a} | 142 ± 16^{a} | 256 ± 18^{b} |

V_{1/2} (mV) | −90 ± 10^{a} | −63 ± 4^{b} | −67 ± 1^{b} |

z_{g} | 0.7 ± 0.1 | 0.7 ± 0.02 | 0.7 ± 0.01 |

τ_{Activation} | 1.3 ± 0.2 | 1.3 ± 0.1 | 1.2 ± 0.4 |

τ_{Deactivation} | 0.1 ± 0.02^{a} | 0.4 ± 0.04^{b} | 0.4 ± 0.1^{b} |

R | 0.2 ± 0.02^{a} | 0.7 ± 0.01^{b} | 0.8 ± 0.1^{b} |

From the fit, the maximal I

_{tail}_{0}(I_{tail}_{0}max), the half-maximal activation voltage (V_{1/2}), and the gating charge (*z*) were derived. The time-dependent current proportion of activating currents and deactivating tail currents were fitted by standard exponential functions to derive activating (τ_{g}_{Activation}) and deactivating (τ_{Deactivation}) time constants. The rectification rates (*R*) were derived by dividing the steady state time-dependent conductance at the deactivating tail voltage of +60 mV by the conductance at the activating voltage of −120 mV. Data are averages ± se. Significant differences were tested for each parameter by ANOVA followed by Holm-Sidak multiple comparison (P ≤ 0.001) and are indicated by different letters.