Table 1. Specific Activities of NSH Enzymes, Enzyme Complexes, and Variants
Specific Activity (µmol min−1 mg−1)
NSHa Xanthosine (0.25 mM) Inosine (0.25 mM)Uridine (0.25 mM)
1-b0.61 ± 0.010c5.7 ± 0.2
-2000
1215.4 ± 0.43.9 ± 0.92.0 ± 0.5
Δ1 -000
Δ126 ± 20c0c
-Δ2000
1Δ20.22 ± 0.0200.4 ± 0.1
  • Errors are sd (n = 3).

  • a NSH1-NSH2 heterocomplexes were always purified via Strep-tagged NSH2 to avoid excess NSH1.

  • b -indicates that only one nucleoside hydrolase was expressed and purified.

  • c Activities were detected at higher substrate concentrations (see Supplemental Table 1).