Table 4. Selectable Promoters in Use in Chlamydomonas Transformation
PromoterExpression TypeOriginReference
RBCS2ConstitutiveChlamydomonas(Stevens et al., 1996)
PsaDConstitutiveChlamydomonas(Fischer and Rochaix, 2001)
HSP70AConstitutive, heat-inducibleChlamydomonas(Schroda et al., 2000)
RBCS2:HSP70AConstitutive, heat-inducibleChlamydomonas(Schroda et al., 2000)
NIT1Ammonium-inducible, nitrogen-repressibleChlamydomonas(Ohresser et al., 1997)
CYC6Nickel-inducible, copper-repressibleChlamydomonas(Pérez-Martín et al., 2015)
THI4aThiamine-repressibleChlamydomonas(Croft et al., 2007)
METEVitamin B12-repressibleChlamydomonas(Helliwell et al., 2014)
35SConstitutiveCaMV(Díaz-Santos et al., 2013)
Nopaline synthase (NOS)ConstitutiveAgrobacterium(Díaz-Santos et al., 2013)
  • a THI4 is a riboswitch found in the THI4 transcript that provides thiamine-repressible control of gene expression when fused to a promoter.