Table 4. Forward Genetic Approaches Using the Tobacco Tnt1 Retrotransposon as a Mutagen
Transformation MethodGenomic Regions Showing InsertionsReferences
PEG-mediated transformation of protoplastsGenerated mutations in genic regions(Vives et al., 2016)
Agrobacterium-mediated transformationGenerated mutations in genic regions and GC-rich regions(Mohanasundaram et al., 2019)