Table 1. Data Collection and Refinement Statistics
ParameterDNA-bound HD1CCT/GHD8/OsNF-YC2GHD8/OsNF-YC2
Space groupP 31 2 1P 61
Cell dimensions
a, b, c (Å)136.08, 136.08, 62.9689.27, 89.27, 81.34
α, β, γ (°)90, 90, 12090, 90, 120
Wavelength (Å)0.979140.97946
Resolution (Å)45 ∼2.55 (2.66∼2.55)45∼2.00 (2.05∼2.00)
Rmerge (%)9.0 (80.0)8.2 (58.0)
Rpim (%)2.9 (26.7)2.6 (18.1)
I/σ14.8 (2.4)21.2(5.3)
Completeness (%)99.5 (99.2)99.7 (98.0)
Number of measured reflections217, 423 (24,970)270, 613 (19,817)
Number of unique reflections22, 041 (2,658)25, 020 (1,825)
Redundancy9.9 (9.4)10.8 (10.9)
Wilson B factor (Å2)45.522.3
Rwork/Rfree (%)19.95/22.5817.06/21.46
Number of atoms
Protein1,7082,737
Main chain8401,356
Side chain8681,381
Water molecules16168
Other entities (DNA)1,0250
All atoms2,7492,905
Average B value (Å2)
Protein67.936.7
Main chain64.631.9
Side chain71.141.5
Water molecules63.046.3
Other entities (DNA)77.70
All atoms71.537.3
Root mean square deviations
Bonds (Å)0.0100.007
Angle (°)1.0940.853
Ramachandran plot statistics (%)
Most favorable96.198.5
Additionally allowed3.91.5
Generously allowed00
  • Values in parentheses are for the highest resolution shell. Rmerge = ΣhΣi|Ih,i-Ih|/ΣhΣiIh,i, where Ih is the mean intensity of the i observations of symmetry-related reflections of h. R = Σ|Fobs Fcalc|/ΣFobs, where Fcalc is the calculated protein structure factor from the atomic model (Rfree was calculated with 5% of the reflections selected).