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Bernoux, M., Timmers, T., Jauneau, A., Brière, C., de Wit, P.J.G.M., Marco, Y., Deslandes, L. (2008). RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector. Plant Cell 20: 2252–2264.
The authors of the above article request the correction of Supplemental Figure 6, as it has come to our attention that the bottom lanes labeled “WB: α-HA” are not correct as indicated, but came from a different immunoblot. This was an inadvertent mistake, as the correct blots look very similar. We apologize for this mistake.
The original figure and a corrected version are shown below. This correction does not change the conclusion of the figure, as the purpose of this immunoblot was to verify the accumulation of intact RD-YFP fusion proteins (RD19, RDL1, and RDL2) in presence or absence of PopP2-3HA protein.
(corrected). Detection of Full Length YFPv-Tagged Proteins.
RD19-YFPv, RDL1-YFPv and RDL2-YFPv were transiently expressed alone or in the presence of PopP2-3HA in N. benthamiana. YFPv-tagged proteins and PopP2-3HA were detected using anti-GFP and anti-PopP2 antibodies, respectively. *Similar amounts of total protein extracts were loaded as indicated by the intensity of non-specific bands detected on each immunoblot.
(original). Detection of Full Length YFPv-Tagged Proteins.
RD19-YFPv (63.8 kD), RDL1-YFPv (68 kD) and RDL2-YFPv (69.6 kD) were transiently expressed alone or in the presence of PopP2-3HA in N. benthamiana. YFPv- and HA-tagged proteins were detected using anti-GFP and anti-HA antibodies, respectively. Equal amounts of total protein extracts were loaded as indicated by Ponceau staining.
The revised figure corresponds to data obtained in 2008, and shows an anti-GFP immunoblot (top) showing the accumulation levels of RD19-YFPv, RDL1-YFPv, and RDL2-YFPv proteins expressed either alone or in the presence of PopP2-3HA, and an anti-PopP2 immunoblot (bottom) showing the accumulation levels of PopP2-3HA from the same protein extracts. Unfortunately, we no longer have the images of Ponceau staining corresponding to these immunoblots. Nevertheless, we believe that the non-specific signals visible on each of the two immunoblots allows us to conclude that similar amounts of protein were loaded in the different lanes, sufficient to make the stated conclusion.
Note: This correction was reviewed by members of The Plant Cell editorial board. The authors are responsible for providing a complete listing and accurate explanations for all known errors or instances of inappropriate data handling or image manipulation associated with the original publication.